Isolation, characterization and biological activity of lactic acid bacteria isolated from Malaysian fermented food and milk products / Nor Zaihana Abdul Rahman

Abdul Rahman, Nor Zaihana (2010) Isolation, characterization and biological activity of lactic acid bacteria isolated from Malaysian fermented food and milk products / Nor Zaihana Abdul Rahman. Masters thesis, Universiti Teknologi MARA.

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Abstract

The use of probiotics to enhance human health has been a considerable interest 'in the recent years and therefore the search for strains with good probiotic characteristics is important. The objectives of this study includes isolation of lactic acid bacteria (LAB) with antibacterial activity from Malaysian fermented food and milk products, identification of the selected LAB and assessment of the probiotic characteristics (tolerance to low pH and high bile concentrations as well as the ability to adhere to Int- 407, a normal intestinal cell line). The LAB were further investigated for cytotoxic, cholesterol reducing and antagonistic activities in vitro. 121 LAB were isolated in the preliminary screening based on inhibitory activity against M. luteus. 22.3 % of the isolates were identified as active, 52.1 % were moderately active and 25.6 % were considered as not active based on the inhibition zones. Twelve of the isolates that showed better antimicrobial activity than the positive control, L. casei strain Shirota (LABPC) in the preliminary screening, were selected for subsequent studies.

Item Type: Thesis (Masters)
Subjects: Q Science > QR Microbiology > Microbial ecology
Divisions: Faculty of Pharmacy
Depositing User: Pn. Zuhaslinda Sulaiman
Date Deposited: 18 Jul 2012 04:33
Last Modified: 18 Jul 2012 04:33
URI: http://eprints.uitm.edu.my/id/eprint/5677

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